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mitotempo 16621  (Cayman Chemical)


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    Structured Review

    Cayman Chemical mitotempo 16621
    Mitotempo 16621, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mitotempo+16621/mitotempo+16621/pm39929434-159-0-6
    Average 90 stars, based on 1 article reviews
    mitotempo 16621 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Transferred mitochondria accumulate reactive oxygen species, promoting proliferation
    Article Snippet: To quench mitochondrial ROS, MitoTEMPO (Cayman Chemical, 16621) was formulated at 200 mM in 100% DMSO and diluted directly into warm complete media for a final concentration of 100 μM.

    Article Title: Inhibitors of Oxidative Phosphorylation Modulate Astrocyte Inflammatory Responses through AMPK-Dependent Ptgs2 mRNA Stabilization
    Article Snippet: Cells were treated for indicated times with 5 ng/ml interleukin-1α (IL-1α) (400-01A, Peprotech, Hamburg, Germany), 20 ng/ml tumor necrosis factor-α (TNFα) (400-14, Peprotech, Hamburg, Germany), 1 μM rotenone (13995, Cayman, Ann Arbor, MI, USA), 2.5 μM oligomycin (11342, Cayman, Ann Arbor, MI, USA), 10 μg/ml antimycin A (A8674, Sigma-Aldrich, Taufkirchen, Germany), 250 μM phenformin (P7045, Sigma-Aldrich, Taufkirchen, Germany), 100 μM A-769662 (3336, Tocris, Minneapolis, MN, USA ), 5 μg/ml actinomycin D (A4262, Sigma-Aldrich, Taufkirchen, Germany), or 100 μM mitoTEMPO (16621, Cayman, Ann Arbor, MI, USA).

    Article Title: Plasma iron controls neutrophil production and function
    Article Snippet: A total of 25 μM DPI (Sigma-Aldrich, D2926) was used to block total ROS generation from ionomycin and PMA, 100 μM apocynin (Abcam, ab120615) was used to block ROS generation via NOX2 complex by PMA, and 5 μM mitoTEMPO (Cayman Chemical, 16621) was used to inhibit mitoROS production.

    Article Title: Traditional medicine Bazi Bushen potentiates immunosurveillance of senescent liver cancer cells via cGAS-STING signaling activation in macrophages.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Ex Vivo:

    Article Title: Transferred mitochondria accumulate reactive oxygen species, promoting proliferation
    Article Snippet: To quench mitochondrial ROS, MitoTEMPO (Cayman Chemical, 16621) was formulated at 200 mM in 100% DMSO and diluted directly into warm complete media for a final concentration of 100 μM.

    Article Title: Inhibitors of Oxidative Phosphorylation Modulate Astrocyte Inflammatory Responses through AMPK-Dependent Ptgs2 mRNA Stabilization
    Article Snippet: Cells were treated for indicated times with 5 ng/ml interleukin-1α (IL-1α) (400-01A, Peprotech, Hamburg, Germany), 20 ng/ml tumor necrosis factor-α (TNFα) (400-14, Peprotech, Hamburg, Germany), 1 μM rotenone (13995, Cayman, Ann Arbor, MI, USA), 2.5 μM oligomycin (11342, Cayman, Ann Arbor, MI, USA), 10 μg/ml antimycin A (A8674, Sigma-Aldrich, Taufkirchen, Germany), 250 μM phenformin (P7045, Sigma-Aldrich, Taufkirchen, Germany), 100 μM A-769662 (3336, Tocris, Minneapolis, MN, USA ), 5 μg/ml actinomycin D (A4262, Sigma-Aldrich, Taufkirchen, Germany), or 100 μM mitoTEMPO (16621, Cayman, Ann Arbor, MI, USA).

    Article Title: Plasma iron controls neutrophil production and function
    Article Snippet: A total of 25 μM DPI (Sigma-Aldrich, D2926) was used to block total ROS generation from ionomycin and PMA, 100 μM apocynin (Abcam, ab120615) was used to block ROS generation via NOX2 complex by PMA, and 5 μM mitoTEMPO (Cayman Chemical, 16621) was used to inhibit mitoROS production.

    Article Title: Traditional medicine Bazi Bushen potentiates immunosurveillance of senescent liver cancer cells via cGAS-STING signaling activation in macrophages.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Isolation:

    Article Title: Transferred mitochondria accumulate reactive oxygen species, promoting proliferation
    Article Snippet: To quench mitochondrial ROS, MitoTEMPO (Cayman Chemical, 16621) was formulated at 200 mM in 100% DMSO and diluted directly into warm complete media for a final concentration of 100 μM.

    Article Title: Inhibitors of Oxidative Phosphorylation Modulate Astrocyte Inflammatory Responses through AMPK-Dependent Ptgs2 mRNA Stabilization
    Article Snippet: Cells were treated for indicated times with 5 ng/ml interleukin-1α (IL-1α) (400-01A, Peprotech, Hamburg, Germany), 20 ng/ml tumor necrosis factor-α (TNFα) (400-14, Peprotech, Hamburg, Germany), 1 μM rotenone (13995, Cayman, Ann Arbor, MI, USA), 2.5 μM oligomycin (11342, Cayman, Ann Arbor, MI, USA), 10 μg/ml antimycin A (A8674, Sigma-Aldrich, Taufkirchen, Germany), 250 μM phenformin (P7045, Sigma-Aldrich, Taufkirchen, Germany), 100 μM A-769662 (3336, Tocris, Minneapolis, MN, USA ), 5 μg/ml actinomycin D (A4262, Sigma-Aldrich, Taufkirchen, Germany), or 100 μM mitoTEMPO (16621, Cayman, Ann Arbor, MI, USA).

    Article Title: Plasma iron controls neutrophil production and function
    Article Snippet: A total of 25 μM DPI (Sigma-Aldrich, D2926) was used to block total ROS generation from ionomycin and PMA, 100 μM apocynin (Abcam, ab120615) was used to block ROS generation via NOX2 complex by PMA, and 5 μM mitoTEMPO (Cayman Chemical, 16621) was used to inhibit mitoROS production.

    Article Title: Traditional medicine Bazi Bushen potentiates immunosurveillance of senescent liver cancer cells via cGAS-STING signaling activation in macrophages.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Fluorescence:

    Article Title: Transferred mitochondria accumulate reactive oxygen species, promoting proliferation
    Article Snippet: To quench mitochondrial ROS, MitoTEMPO (Cayman Chemical, 16621) was formulated at 200 mM in 100% DMSO and diluted directly into warm complete media for a final concentration of 100 μM.

    Article Title: Inhibitors of Oxidative Phosphorylation Modulate Astrocyte Inflammatory Responses through AMPK-Dependent Ptgs2 mRNA Stabilization
    Article Snippet: Cells were treated for indicated times with 5 ng/ml interleukin-1α (IL-1α) (400-01A, Peprotech, Hamburg, Germany), 20 ng/ml tumor necrosis factor-α (TNFα) (400-14, Peprotech, Hamburg, Germany), 1 μM rotenone (13995, Cayman, Ann Arbor, MI, USA), 2.5 μM oligomycin (11342, Cayman, Ann Arbor, MI, USA), 10 μg/ml antimycin A (A8674, Sigma-Aldrich, Taufkirchen, Germany), 250 μM phenformin (P7045, Sigma-Aldrich, Taufkirchen, Germany), 100 μM A-769662 (3336, Tocris, Minneapolis, MN, USA ), 5 μg/ml actinomycin D (A4262, Sigma-Aldrich, Taufkirchen, Germany), or 100 μM mitoTEMPO (16621, Cayman, Ann Arbor, MI, USA).

    Article Title: Plasma iron controls neutrophil production and function
    Article Snippet: A total of 25 μM DPI (Sigma-Aldrich, D2926) was used to block total ROS generation from ionomycin and PMA, 100 μM apocynin (Abcam, ab120615) was used to block ROS generation via NOX2 complex by PMA, and 5 μM mitoTEMPO (Cayman Chemical, 16621) was used to inhibit mitoROS production.

    Article Title: Traditional medicine Bazi Bushen potentiates immunosurveillance of senescent liver cancer cells via cGAS-STING signaling activation in macrophages.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Labeling:

    Article Title: Transferred mitochondria accumulate reactive oxygen species, promoting proliferation
    Article Snippet: To quench mitochondrial ROS, MitoTEMPO (Cayman Chemical, 16621) was formulated at 200 mM in 100% DMSO and diluted directly into warm complete media for a final concentration of 100 μM.

    Article Title: Inhibitors of Oxidative Phosphorylation Modulate Astrocyte Inflammatory Responses through AMPK-Dependent Ptgs2 mRNA Stabilization
    Article Snippet: Cells were treated for indicated times with 5 ng/ml interleukin-1α (IL-1α) (400-01A, Peprotech, Hamburg, Germany), 20 ng/ml tumor necrosis factor-α (TNFα) (400-14, Peprotech, Hamburg, Germany), 1 μM rotenone (13995, Cayman, Ann Arbor, MI, USA), 2.5 μM oligomycin (11342, Cayman, Ann Arbor, MI, USA), 10 μg/ml antimycin A (A8674, Sigma-Aldrich, Taufkirchen, Germany), 250 μM phenformin (P7045, Sigma-Aldrich, Taufkirchen, Germany), 100 μM A-769662 (3336, Tocris, Minneapolis, MN, USA ), 5 μg/ml actinomycin D (A4262, Sigma-Aldrich, Taufkirchen, Germany), or 100 μM mitoTEMPO (16621, Cayman, Ann Arbor, MI, USA).

    Article Title: Plasma iron controls neutrophil production and function
    Article Snippet: A total of 25 μM DPI (Sigma-Aldrich, D2926) was used to block total ROS generation from ionomycin and PMA, 100 μM apocynin (Abcam, ab120615) was used to block ROS generation via NOX2 complex by PMA, and 5 μM mitoTEMPO (Cayman Chemical, 16621) was used to inhibit mitoROS production.

    Article Title: Traditional medicine Bazi Bushen potentiates immunosurveillance of senescent liver cancer cells via cGAS-STING signaling activation in macrophages.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Produced:

    Article Title: Transferred mitochondria accumulate reactive oxygen species, promoting proliferation
    Article Snippet: To quench mitochondrial ROS, MitoTEMPO (Cayman Chemical, 16621) was formulated at 200 mM in 100% DMSO and diluted directly into warm complete media for a final concentration of 100 μM.

    Article Title: Inhibitors of Oxidative Phosphorylation Modulate Astrocyte Inflammatory Responses through AMPK-Dependent Ptgs2 mRNA Stabilization
    Article Snippet: Cells were treated for indicated times with 5 ng/ml interleukin-1α (IL-1α) (400-01A, Peprotech, Hamburg, Germany), 20 ng/ml tumor necrosis factor-α (TNFα) (400-14, Peprotech, Hamburg, Germany), 1 μM rotenone (13995, Cayman, Ann Arbor, MI, USA), 2.5 μM oligomycin (11342, Cayman, Ann Arbor, MI, USA), 10 μg/ml antimycin A (A8674, Sigma-Aldrich, Taufkirchen, Germany), 250 μM phenformin (P7045, Sigma-Aldrich, Taufkirchen, Germany), 100 μM A-769662 (3336, Tocris, Minneapolis, MN, USA ), 5 μg/ml actinomycin D (A4262, Sigma-Aldrich, Taufkirchen, Germany), or 100 μM mitoTEMPO (16621, Cayman, Ann Arbor, MI, USA).

    Article Title: Plasma iron controls neutrophil production and function
    Article Snippet: A total of 25 μM DPI (Sigma-Aldrich, D2926) was used to block total ROS generation from ionomycin and PMA, 100 μM apocynin (Abcam, ab120615) was used to block ROS generation via NOX2 complex by PMA, and 5 μM mitoTEMPO (Cayman Chemical, 16621) was used to inhibit mitoROS production.

    Article Title: Traditional medicine Bazi Bushen potentiates immunosurveillance of senescent liver cancer cells via cGAS-STING signaling activation in macrophages.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Transferred mitochondria accumulate reactive oxygen species, promoting proliferation
    Article Snippet: To quench mitochondrial ROS, MitoTEMPO (Cayman Chemical, 16621) was formulated at 200 mM in 100% DMSO and diluted directly into warm complete media for a final concentration of 100 μM.

    Article Title: Inhibitors of Oxidative Phosphorylation Modulate Astrocyte Inflammatory Responses through AMPK-Dependent Ptgs2 mRNA Stabilization
    Article Snippet: Cells were treated for indicated times with 5 ng/ml interleukin-1α (IL-1α) (400-01A, Peprotech, Hamburg, Germany), 20 ng/ml tumor necrosis factor-α (TNFα) (400-14, Peprotech, Hamburg, Germany), 1 μM rotenone (13995, Cayman, Ann Arbor, MI, USA), 2.5 μM oligomycin (11342, Cayman, Ann Arbor, MI, USA), 10 μg/ml antimycin A (A8674, Sigma-Aldrich, Taufkirchen, Germany), 250 μM phenformin (P7045, Sigma-Aldrich, Taufkirchen, Germany), 100 μM A-769662 (3336, Tocris, Minneapolis, MN, USA ), 5 μg/ml actinomycin D (A4262, Sigma-Aldrich, Taufkirchen, Germany), or 100 μM mitoTEMPO (16621, Cayman, Ann Arbor, MI, USA).

    Article Title: Plasma iron controls neutrophil production and function
    Article Snippet: A total of 25 μM DPI (Sigma-Aldrich, D2926) was used to block total ROS generation from ionomycin and PMA, 100 μM apocynin (Abcam, ab120615) was used to block ROS generation via NOX2 complex by PMA, and 5 μM mitoTEMPO (Cayman Chemical, 16621) was used to inhibit mitoROS production.

    Article Title: Traditional medicine Bazi Bushen potentiates immunosurveillance of senescent liver cancer cells via cGAS-STING signaling activation in macrophages.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Microscopy:

    Article Title: Transferred mitochondria accumulate reactive oxygen species, promoting proliferation
    Article Snippet: To quench mitochondrial ROS, MitoTEMPO (Cayman Chemical, 16621) was formulated at 200 mM in 100% DMSO and diluted directly into warm complete media for a final concentration of 100 μM.

    Article Title: Inhibitors of Oxidative Phosphorylation Modulate Astrocyte Inflammatory Responses through AMPK-Dependent Ptgs2 mRNA Stabilization
    Article Snippet: Cells were treated for indicated times with 5 ng/ml interleukin-1α (IL-1α) (400-01A, Peprotech, Hamburg, Germany), 20 ng/ml tumor necrosis factor-α (TNFα) (400-14, Peprotech, Hamburg, Germany), 1 μM rotenone (13995, Cayman, Ann Arbor, MI, USA), 2.5 μM oligomycin (11342, Cayman, Ann Arbor, MI, USA), 10 μg/ml antimycin A (A8674, Sigma-Aldrich, Taufkirchen, Germany), 250 μM phenformin (P7045, Sigma-Aldrich, Taufkirchen, Germany), 100 μM A-769662 (3336, Tocris, Minneapolis, MN, USA ), 5 μg/ml actinomycin D (A4262, Sigma-Aldrich, Taufkirchen, Germany), or 100 μM mitoTEMPO (16621, Cayman, Ann Arbor, MI, USA).

    Article Title: Plasma iron controls neutrophil production and function
    Article Snippet: A total of 25 μM DPI (Sigma-Aldrich, D2926) was used to block total ROS generation from ionomycin and PMA, 100 μM apocynin (Abcam, ab120615) was used to block ROS generation via NOX2 complex by PMA, and 5 μM mitoTEMPO (Cayman Chemical, 16621) was used to inhibit mitoROS production.

    Article Title: Traditional medicine Bazi Bushen potentiates immunosurveillance of senescent liver cancer cells via cGAS-STING signaling activation in macrophages.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Inhibition:

    Article Title: Transferred mitochondria accumulate reactive oxygen species, promoting proliferation
    Article Snippet: To quench mitochondrial ROS, MitoTEMPO (Cayman Chemical, 16621) was formulated at 200 mM in 100% DMSO and diluted directly into warm complete media for a final concentration of 100 μM.

    Article Title: Inhibitors of Oxidative Phosphorylation Modulate Astrocyte Inflammatory Responses through AMPK-Dependent Ptgs2 mRNA Stabilization
    Article Snippet: Cells were treated for indicated times with 5 ng/ml interleukin-1α (IL-1α) (400-01A, Peprotech, Hamburg, Germany), 20 ng/ml tumor necrosis factor-α (TNFα) (400-14, Peprotech, Hamburg, Germany), 1 μM rotenone (13995, Cayman, Ann Arbor, MI, USA), 2.5 μM oligomycin (11342, Cayman, Ann Arbor, MI, USA), 10 μg/ml antimycin A (A8674, Sigma-Aldrich, Taufkirchen, Germany), 250 μM phenformin (P7045, Sigma-Aldrich, Taufkirchen, Germany), 100 μM A-769662 (3336, Tocris, Minneapolis, MN, USA ), 5 μg/ml actinomycin D (A4262, Sigma-Aldrich, Taufkirchen, Germany), or 100 μM mitoTEMPO (16621, Cayman, Ann Arbor, MI, USA).

    Article Title: Plasma iron controls neutrophil production and function
    Article Snippet: A total of 25 μM DPI (Sigma-Aldrich, D2926) was used to block total ROS generation from ionomycin and PMA, 100 μM apocynin (Abcam, ab120615) was used to block ROS generation via NOX2 complex by PMA, and 5 μM mitoTEMPO (Cayman Chemical, 16621) was used to inhibit mitoROS production.

    Article Title: Traditional medicine Bazi Bushen potentiates immunosurveillance of senescent liver cancer cells via cGAS-STING signaling activation in macrophages.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.



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    Mitochondrial DNA released into the cytoplasm by H 2 O 2 stimulation is involved in MUC5AC transcription induction. (A) Images of immunofluorescence staining of mitochondria of NCI‐H292 with anti‐TOM20 antibody when stimulated with H 2 O 2 for 24 h. (left) No stimulation, (right) H 2 O 2 100 μM. Green: mitochondria (anti‐TOM20 antibody); Blue: DAPI. Original magnification, 400×; bar = 15 μm. (B) The amount of mtDNA (NAD1) in the cytoplasm at 1 and 6 h after 100 μM H 2 O 2 stimulation ( n = 4). (C) MUC5AC mRNA in NCI‐H292 cells pretreated with <t>MitoTEMPO</t> (0, 30, 100, and 300 μM) and stimulated with 100 μM H 2 O 2 for 24 h ( n = 4). (D) MUC5AC mRNA in NCI‐H292 cells treated with 0–5 μM Thapsigargin for 4 h ( n = 4). The results are represented as mean ± SD. Significant differences are indicated by ** p < 0.01. Significance was calculated using the Dunnett's test (B–D).
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    Mitochondrial DNA released into the cytoplasm by H 2 O 2 stimulation is involved in MUC5AC transcription induction. (A) Images of immunofluorescence staining of mitochondria of NCI‐H292 with anti‐TOM20 antibody when stimulated with H 2 O 2 for 24 h. (left) No stimulation, (right) H 2 O 2 100 μM. Green: mitochondria (anti‐TOM20 antibody); Blue: DAPI. Original magnification, 400×; bar = 15 μm. (B) The amount of mtDNA (NAD1) in the cytoplasm at 1 and 6 h after 100 μM H 2 O 2 stimulation ( n = 4). (C) MUC5AC mRNA in NCI‐H292 cells pretreated with <t>MitoTEMPO</t> (0, 30, 100, and 300 μM) and stimulated with 100 μM H 2 O 2 for 24 h ( n = 4). (D) MUC5AC mRNA in NCI‐H292 cells treated with 0–5 μM Thapsigargin for 4 h ( n = 4). The results are represented as mean ± SD. Significant differences are indicated by ** p < 0.01. Significance was calculated using the Dunnett's test (B–D).
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    Image Search Results


    Mitochondrial DNA released into the cytoplasm by H 2 O 2 stimulation is involved in MUC5AC transcription induction. (A) Images of immunofluorescence staining of mitochondria of NCI‐H292 with anti‐TOM20 antibody when stimulated with H 2 O 2 for 24 h. (left) No stimulation, (right) H 2 O 2 100 μM. Green: mitochondria (anti‐TOM20 antibody); Blue: DAPI. Original magnification, 400×; bar = 15 μm. (B) The amount of mtDNA (NAD1) in the cytoplasm at 1 and 6 h after 100 μM H 2 O 2 stimulation ( n = 4). (C) MUC5AC mRNA in NCI‐H292 cells pretreated with MitoTEMPO (0, 30, 100, and 300 μM) and stimulated with 100 μM H 2 O 2 for 24 h ( n = 4). (D) MUC5AC mRNA in NCI‐H292 cells treated with 0–5 μM Thapsigargin for 4 h ( n = 4). The results are represented as mean ± SD. Significant differences are indicated by ** p < 0.01. Significance was calculated using the Dunnett's test (B–D).

    Journal: FASEB BioAdvances

    Article Title: Oxidative stress induces MUC5AC expression through mitochondrial damage‐dependent STING signaling in human bronchial epithelial cells

    doi: 10.1096/fba.2022-00081

    Figure Lengend Snippet: Mitochondrial DNA released into the cytoplasm by H 2 O 2 stimulation is involved in MUC5AC transcription induction. (A) Images of immunofluorescence staining of mitochondria of NCI‐H292 with anti‐TOM20 antibody when stimulated with H 2 O 2 for 24 h. (left) No stimulation, (right) H 2 O 2 100 μM. Green: mitochondria (anti‐TOM20 antibody); Blue: DAPI. Original magnification, 400×; bar = 15 μm. (B) The amount of mtDNA (NAD1) in the cytoplasm at 1 and 6 h after 100 μM H 2 O 2 stimulation ( n = 4). (C) MUC5AC mRNA in NCI‐H292 cells pretreated with MitoTEMPO (0, 30, 100, and 300 μM) and stimulated with 100 μM H 2 O 2 for 24 h ( n = 4). (D) MUC5AC mRNA in NCI‐H292 cells treated with 0–5 μM Thapsigargin for 4 h ( n = 4). The results are represented as mean ± SD. Significant differences are indicated by ** p < 0.01. Significance was calculated using the Dunnett's test (B–D).

    Article Snippet: Cells were stimulated with 2′3′ cGAMP for 16 h. MitoTEMPO (Cayman Chemical, 16621) was preadministered to cells 1 h before H 2 O 2 stimulation.

    Techniques: Immunofluorescence, Staining

    Mitochondrial damage and STING are involved in H 2 O 2 ‐stimulated MUC5AC transcriptional activity in NHBE cells. MUC5AC mRNA expression upon H 2 O 2 stimulation in NHBE cells pretreated with 200 μM MitoTEMPO and 2 μM STING inhibitor H‐151 ( n = 8). Significant differences are indicated by p < 0.01. Significance was calculated using the Dunnett's test.

    Journal: FASEB BioAdvances

    Article Title: Oxidative stress induces MUC5AC expression through mitochondrial damage‐dependent STING signaling in human bronchial epithelial cells

    doi: 10.1096/fba.2022-00081

    Figure Lengend Snippet: Mitochondrial damage and STING are involved in H 2 O 2 ‐stimulated MUC5AC transcriptional activity in NHBE cells. MUC5AC mRNA expression upon H 2 O 2 stimulation in NHBE cells pretreated with 200 μM MitoTEMPO and 2 μM STING inhibitor H‐151 ( n = 8). Significant differences are indicated by p < 0.01. Significance was calculated using the Dunnett's test.

    Article Snippet: Cells were stimulated with 2′3′ cGAMP for 16 h. MitoTEMPO (Cayman Chemical, 16621) was preadministered to cells 1 h before H 2 O 2 stimulation.

    Techniques: Activity Assay, Expressing